Chloramphenicol resistance selection in Bacillus licheniformis
US8993304B2 · kind B2 · utility
0Cited by
1References
20Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Apr 2, 2013 |
| Grant date | Mar 31, 2015 |
| Priority date | — |
| Expiry date | Apr 2, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1033
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a modified Bacillus licheniformis host cell, wherein one or more naturally occurring chromosomal chloramphenicol acetyl transferase encoding gene(s), cat, has been inactivated. The inactivation of the chromosomal cat gene(s) allows the use of chloramphenicol as an efficient selective agent in methods for DNA introduction into the modified cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.