Methods for detection and analysis of polynucleotide-binding protein interactions
US8993335B2 · kind B2 · utility
54Cited by
29References
17Claims
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Key dates
| Filing date | Feb 13, 2004 |
| Grant date | Mar 31, 2015 |
| Priority date | — |
| Expiry date | Oct 19, 2026 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor PBP that can bind to the target polynucleotide. The sensor PBP comprises a signaling chromophore to absorb energy from the excited multichromophore and emit light in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.
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