Microscopy system and method for creating three dimensional images using probe molecules

US8994807B2 · kind B2 · utility

Assignees

• University of Utah Research Foundation · US
• University of Maine System Board of Trustees · US
• The Jackson Laboratory · US

Inventors

• Brian T. Bennett · Longboat Key, US
• Joerg Bewersdorf · Bar Harbor, US
• Erik M. Jorgensen · Salt Lake City, US
• Sam T. Hess · Orono, US
• Travis Gould · Old Town, US
• Mudalige Siyath Gunewardene · Orono, US

Key dates

• Filing date: Mar 18, 2010
• Grant date: Mar 31, 2015
• Expiry date: Jul 28, 2032

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B21/22
• WIPO field: Optics
• WIPO sector: Instruments

Abstract

A system (100) and method for creating three dimensional images using probe molecules is disclosed and described. A sample is mounted on a stage (160). The sample has a plurality of probe molecules. The sample is illuminated with light, causing the probe molecules to luminesce. The probe luminescence can be split into at least four paths corresponding to at least four detection planes corresponding to object planes in the sample. The at least four detection planes are detected via a camera (155). Object planes in corresponding recorded regions of interest are recorded in the camera (155). A signal from the regions of interest is combined into a three dimensional image.