Patent · US Active

Helicase dependent isothermal amplification using nicking enzymes

US9005933B2 · kind B2 · utility

0Cited by

5References

19Claims

0Family size

Assignee

QIAGEN GmbH · DE

Inventors

Christian Korfhage · Hilden, DE
Gerd Grosshauser · Pulheim, DE
Thomas Rothmann · Langenfeld, DE
Ralf Himmelreich · Langenfeld, DE

Key dates

Filing date	Aug 16, 2011
Grant date	Apr 14, 2015
Priority date	—
Expiry date	Dec 18, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6844
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.