High-throughput biological screening

US9006149B2 · kind B2 · utility

Assignees

• The Charles Stark Draper Laboratory, Inc. · US
• The Brigham and Women's Hospital, Inc. · US

Inventors

• Guillermo Garcia-Cardena · Boston, US
• Peter Mack · Chapel Hill, US
• Jeffrey T. Borenstein · Holliston, US
• Ahmad S. Khalil · Lexington, US
• Eli Weinberg · Needham, US
• Jason O. Fiering · Boston, US
• Ernest S. Kim · Cambridge, US
• William James Louis Adams · DeLand, US

Key dates

• Filing date: Jan 14, 2010
• Grant date: Apr 14, 2015
• Expiry date: Jan 14, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12M35/04
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A high-throughput flow system includes an array of wells and a separate mechanical tip positioned within each well. Each mechanical tip is separately actuated to impart a shear stress pattern. A separate sleeve may be associated with each tip for maintaining a predetermined distance between the tip and a floor of the tip's corresponding well, with each tip being rotatable within its corresponding sleeve. Alternatively, a separate post may be associated with each tip for maintaining a predetermined distance between the tip and a floor of the tip's corresponding well, with each tip being rotatable about its corresponding post.