Device and method for determining the concentration of fluorophores in a sample

US9006685B2 · kind B2 · utility

Assignee

• LRE Medical GmbH · DE

Inventors

• Rafael Egger · Munich, DE
• Jörg Zeiner · Birkenfeld, DE

Key dates

• Filing date: Apr 11, 2012
• Grant date: Apr 14, 2015
• Expiry date: Jun 17, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2201/068
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A device (10) and a method for analyzing a sample (16) containing fluorophores use a light source (12) emitting light (λex) onto the sample (16), and onto a fluorescence standard (14). The fluorophores of the sample (16), given an immission of light of a first wavelength (λex1), have a first excitation efficiency and, given an immission of light of a second wavelength (λex2), have a second excitation efficiency. The fluorescence standard (14), given the same immissions of light, has a third excitation efficiency and, a fourth excitation efficiency. An optical element (20) which is arranged between the light source (12) and the sample (16) and/or (12) the fluorescence standard (14) adapts, due to its optical property, a first difference between the first excitation efficiency and the second excitation efficiency and a second difference between the third excitation efficiency and the fourth excitation efficiency to each other.