Cell based screening assays for the triglyceride synthesis pathway
US9012159B2 · kind B2 · utility
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Key dates
| Filing date | Jan 7, 2010 |
| Grant date | Apr 21, 2015 |
| Priority date | — |
| Expiry date | Jun 20, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/92
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for identifying a compound which modulates the activity of acyl-coA: diacylglycerol acyltransferase comprises the steps of contacting a stable isotope labeled fatty acid with cells in either presence or absence of the compound, extracting the cells with isopropyl alcohol, and determining the level of a stable isotope labeled triglyceride in the presence or absence of the compound; wherein a change in the level of the stable isotope labeled triglyceride indicates that the compound modulates the DGAT activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.