Use of copper and glutamate in cell culture for production of polypeptides
US9012180B2 · kind B2 · utility
2Cited by
19References
31Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 29, 2008 |
| Grant date | Apr 21, 2015 |
| Priority date | — |
| Expiry date | May 20, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improved system for large scale production of polypeptides in cell culture is provided. In accordance with the present invention, cells expressing a polypeptide of interest are grown in media that contain copper, glutamate or both. The use of such a system allows production of polypeptides in which misfolding and/or aggregation are reduced, and in which total glycosylation is increased. Polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, agricultural or other commercial compositions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.