Look-through mutagenesis for developing altered polypeptides with enhanced properties
US9012369B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jul 6, 2005 |
| Grant date | Apr 21, 2015 |
| Priority date | — |
| Expiry date | Jun 29, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/92
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of mutagenesis by which a predetermined amino acid is introduced into each and every position of a selected set of positions in a preselected region (or several different regions) of a polypeptide to produce a library of polypeptide analogs is disclosed. The method is based on the premise that certain amino acids play a crucial role in the structure and function of proteins and thus is capable of identifying and distinguishing functional amino acid residues (“hot spots”) from non-functional amino acids residues (“cold spots”) within a polypeptide or portion thereof. Libraries can be generated which contain only desired polypeptide analogs and are of reasonable size for screening. The libraries can be used to study the role of specific amino acids in polypeptide structure and function and to develop new or improved polypeptides such as antibodies, antibody fragments, single chain antibodies, enzymes, and ligands.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.