Mixtures of binding proteins
US9012371B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 5, 2011 |
| Grant date | Apr 21, 2015 |
| Priority date | — |
| Expiry date | Oct 5, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2318/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Described are methods for producing libraries of cells expressing at least two separate single polypeptide chain binding proteins, in which the binding proteins have different target epitopes. Such libraries are made by integration of the nucleic acid sequences encoding the polypeptide chains into the genome of the host cell, and selecting for cells that have successfully integrated these nucleic acids. The selected cells are preferably subjected to a cloning step. Mixtures of binding proteins are produced without having to individually produce each of the components of the mixture. A library of cells wherein essentially each cell encodes at least two single polypeptide chain binding proteins having different target epitopes is also herewith provided, as well as methods for producing a composition comprising at least two separate single polypeptide chain binding proteins having different target epitopes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.