Rapid nucleic acid purification using paper chromatography
US9012614B2 · kind B2 · utility
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Key dates
| Filing date | Dec 27, 2011 |
| Grant date | Apr 21, 2015 |
| Priority date | — |
| Expiry date | May 10, 2032 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/255
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided is a method for rapid nucleic acid purification. The method includes allowing the nucleic acid isolated from the biological sample to be adsorbed on a silica membrane; separating contaminants from the nucleic acid-adsorbed silica membrane by using paper chromatography; and eluting only high-purity nucleic acid from the silica membrane from which the contaminants are isolated by using a buffer, or applying at least one selected from direct PCR, RT-PCR, real-time PCR, and real-time RT-PCR to the nucleic acid bound onto the silica membrane.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.