Methods for enzymatic decolorization of chlorophyll

US9017990B2 · kind B2 · utility

Assignee

• DSM IP ASSETS B.V. · NL

Inventors

• David Lam · Lomita, US
• David Weiner · Echt, NL
• Timothy Hitchman · Encinitas, US
• Nelson R. Barton · San Diego, US
• Mark J. Burk · Cambridge, GB

Key dates

• Filing date: May 9, 2011
• Grant date: Apr 28, 2015
• Expiry date: Feb 24, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/18
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides the invention provides compositions and methods for the enzymatic treatment (“bleaching” or “de-colorizing”) of chlorophyll-comprising compositions, e.g., algae preparations, chlorophyll-containing or chlorophyll-contaminated feeds, foods or oils, for example, vegetable oils, including oils processed from oilseeds, such as canola (rapeseed) oil or soybean oil, or oil fruits, such as palm oil. In one aspect, the invention provides methods using a chlorophyllase enzyme for the enzymatic hydrolysis of chlorophyll in an algae, an animal (e.g., a fish) or plant preparation, a food or an oil. In one aspect, the chlorophyllase is immobilized onto a silica. The invention also provides compositions of manufacture and detergents.