System and methods for making and processing emulsions
US9017993B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 9, 2012 |
| Grant date | Apr 28, 2015 |
| Priority date | — |
| Expiry date | Nov 12, 2032 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L3/502784
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.