Method for identification and purification of multi-specific polypeptides
US9018137B2 · kind B2 · utility
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Key dates
| Filing date | Mar 15, 2012 |
| Grant date | Apr 28, 2015 |
| Priority date | — |
| Expiry date | Mar 15, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/468
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The document pertains to a method for the purification of a ternary mixture of dimeric antibodies of the type AA, AB, BB, characterized in that for the separation of the three components and in particular for the isolation of the multi-specific fraction AB multicolumn counter current solvent gradient purification chromatography with a stationary phase load of more than 1 mg antibody mixture per milliliter stationary phase is used. It furthermore relates to a method for the identification of in particular bispecific antibody systems, which are particularly suitable for the application of such a purification method.
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