Isolation and purification of antibodies using protein a affinity chromatography
US9018361B2 · kind B2 · utility
25Cited by
42References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 26, 2014 |
| Grant date | Apr 28, 2015 |
| Priority date | — |
| Expiry date | Mar 26, 2034 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/824
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein are methods for the isolation and purification of antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography, preferably Protein A affinity, ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.