Hydrogen peroxide sensors based upon photo-induced electron transfer
US9018377B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 22, 2010 |
| Grant date | Apr 28, 2015 |
| Priority date | — |
| Expiry date | Aug 18, 2031 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/206664
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The invention provides compounds of formula I F-L-Q (I) where F comprises a fluorophore capable of absorbing energy at an excitation wavelength and, in the absence of a quencher, emitting energy at an emission wavelength, which is different than the excitation wavelength; Q comprises a quencher; L comprises a linker moiety having two ends, one end being covalently bound to F and the other end being covalently bound to Q. The compounds are capable of undergoing a reversible reaction (1), provided below: (1) where Q+ is an oxidized form of Q representing the absence of a quencher, Ox comprises an oxidizing agent, which is capable of oxidizing Q to its oxidized form Q, and Red comprises a reducing agent, which is capable of converting Q back to its reduced form Q. The compounds can undergo photo-induced electron transfer when irradiated with energy and when Q exists in its oxidized form, Q+. The invention also provides methods of detecting and determining the presence of analytes and/or hydrogen peroxide in a sample, as well as a substrate that comprises the compound of formula I.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.