Enhanced citric acid production in Aspergillus with inactivated asparagine-linked glycosylation protein 3 (Alg3), and/or increased LaeA expression
US9023637B2 · kind B2 · utility
2Cited by
1References
20Claims
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Key dates
| Filing date | Nov 30, 2012 |
| Grant date | May 5, 2015 |
| Priority date | — |
| Expiry date | May 25, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y204/01258
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (Lae), or both. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also provided, as are compositions and kits including the disclosed fungi.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.