Patent · US Active

Method for auxiliary identification of inbred line of wuzhishan miniature pig and its special primer

US9029090B2 · kind B2 · utility

0Cited by
1References
5Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMar 1, 2010
Grant dateMay 12, 2015
Priority date
Expiry dateMar 1, 2030

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2600/124
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A method for auxiliary identification of WZSP inbred line and its special primers are disclosed. The method may comprise: testing whether a deoxyribose nucleotide at site 1273 from 5′ end of genomic DNA comprising SEQ ID NO: 1 in a test pig is A or G, measuring whether the test pig genotype is GG, GA or AA wherein the test pig with GG genotype is a candidate for the WZSP inbred line and the test pigs with GA genotype and AA genotype are non-WZSP inbred line. The method can be applied to breed WZSP inbred line, which can be used to preliminarily screen all the pigs in test pig group, eliminate non-WZSP inbred line, find out a candidate WZSP inbred line and make a further confirmation in combination with other methods. The method and primers can also be used to judge whether a WZSP on market is counterfeiting or not.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.