Methods for improved DNA release from binding substrates and/or decreasing PCR inhibition in pathogen detection

US9029529B2 · kind B2 · utility

Assignee

• E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

• T. Joseph Dennes · Parkesburg, US
• Michael P. Perry · Downingtown, US

Key dates

• Filing date: Mar 14, 2012
• Grant date: May 12, 2015
• Expiry date: Jan 20, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1017
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed herein are processes for collecting nucleic acids from particulate samples. One embodiment disclosed herein relates to the use of ultrasonic energy to simultaneously shear large nucleic acid molecules and large particulates to very small sizes prior to or during a chemical binding step to a nucleic acid binding surface. Another embodiment involves crushing the nucleic acid binding surface prior to eluting the bound nucleic acid molecules to enable better wetting of the nucleic acid binding surface and easier diffusion of bound nucleic acid molecules out of the nucleic acid binding surface.