Detection and quantification of hydroxymethylated nucleotides in a polynucleotide preparation

US9034597B2 · kind B2 · utility

Assignee

• New England Biolabs, Inc. · US

Inventors

• Jurate Bitinaite · Rowley, US
• Romualdas Vaisvila · Ipswich, US
• Sriharsa Pradhan · Ipswich, US
• Yu Zheng · Covina, US
• Richard Roberts · Rennes, FR
• Devora Cohen-Karni · Cambridge, US
• Christopher Noren · Boxford, US
• Elisabeth A. Raleigh · Somerville, US
• Geoffrey G. Wilson · Boxford, US
• Hang-Gyeong Chin · Ipswich, US

Key dates

• Filing date: Aug 25, 2010
• Grant date: May 19, 2015
• Expiry date: Nov 2, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/154
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and compositions are described for detecting hydroxymethylated nucleotides (hmNs) in a polynucleotide preparation with a view to mapping the location of hmNs in a genome, quantifying the occurrence of hmNs at selected loci and correlating the occurrence of hmNs with gene expression and phenotypic traits. Embodiments describe the use of modifying enzymes together with site-specific endonucleases to detect the hmNs.