Patent · US Active

Thermocycler and sample vessel for rapid amplification of DNA

US9034635B2 · kind B2 · utility

18Cited by
52References
24Claims
0Family size

Assignee

Inventors

Key dates

Filing dateFeb 19, 2009
Grant dateMay 19, 2015
Priority date
Expiry dateDec 28, 2030

Classification

  • Technology area (CPC B)Performing Operations; Transporting
  • CPC primaryB01L2300/1844
  • WIPO fieldChemical engineering
  • WIPO sectorChemistry

Abstract

A thermocycler apparatus and method for rapidly performing the PCR process employs at least two thermoelectric modules which are in substantial spatial opposition with an interior space present between opposing modules. One or multiple sample vessels are placed in between the modules such that the vessels are subjected to temperature cycling by the modules. The sample vessels have a minimal internal dimension that is substantially perpendicular to the modules that facilitates rapid temperature cycling. In embodiments of the invention the sample vessels may be deformable between: a) a shape having a wide mouth to facilitate filling and removing of sample fluids from the vessel, and b) a shape which is thinner for conforming to the sample cavity or interior space between the thermoelectric modules of the thermocycler for more rapid heat transfer.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.