Protein production method

US9034649B2 · kind B2 · utility

Assignees

• Inter-University Research Institute Corporation Research Organization of Information and Systems · JP
• KYOWA HAKKO KIRIN CO., LTD. · JP

Inventors

• Koichi Kawakami · Mishima, JP
• Keina YAMAGUCHI · Takasaki, JP
• Risa Ogawa · Takasaki, JP
• Masayoshi TSUKAHARA · Shibukawa, JP

Key dates

• Filing date: Jun 11, 2010
• Grant date: May 19, 2015
• Expiry date: Mar 19, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2840/20
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.