Method for enhancing recombinant protein production by cell-free protein expression system
US9040253B2 · kind B2 · utility
3Cited by
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4Claims
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Key dates
| Filing date | Apr 14, 2011 |
| Grant date | May 26, 2015 |
| Priority date | — |
| Expiry date | Sep 2, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07006
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a method for monitoring of profile changes of components in a dynamic system such as a cell-free in vitro protein synthesis system by using liquid chromatography (LC) combined with mass spectrometry (MS). In an additional aspect, this invention provides a method for enhancing the yield and/or reproducibility in a cell-free protein synthesis system by modulating the level and/or activity of a protein component that has regulatory effects on the system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.