Oxidoreductases for the stereoselective reduction of keto compounds

US9040265B2 · kind B2 · utility

Assignee

• IEP GMBH · US

Inventors

• Anke Tschentscher · Eltville am Rhein, DE
• Antje Gupta · Wiesbaden, DE
• Maria Bobkova · Idstein, DE

Key dates

• Filing date: Oct 25, 2011
• Grant date: May 26, 2015
• Expiry date: Dec 30, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P7/42
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a process for the enantioselective enzymatic reduction of a keto compound to the corresponding chiral hydroxy compound, wherein the keto compound is reduced with an oxidoreductase in the presence of a cofactor, and is characterized in that an oxidoreductase is used which has an amino acid sequence in which (a) at least 70% of the amino acids are identical to the amino acids of one of the amino acid sequences SEQ ID No 1, SEQ ID No 6 and SEQ ID No 8, or (b) at least 55% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 2, or (c) at least 65% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 3, or (d) at least 75% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 4, or (e) at least 65% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 5, or (0 at least 50% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 7, or (g) at least 72% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 129.