Metabolically engineered cells for the production of resveratrol or an oligomeric or glycosidically-bound derivative thereof

US9040269B2 · kind B2 · utility

Assignee

• Evola SA · CH

Inventors

• Michael Katz · Malmö, SE
• Hans Peter Smits · Paseo Los Muchachos, DK
• Jochen Förster · Copenhagen, DK
• Jens Nielsen · Copenhagen, DK

Key dates

• Filing date: Apr 17, 2008
• Grant date: May 26, 2015
• Expiry date: Jun 22, 2029

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/52
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.