Methods of expanding embryonic stem cells in a suspension culture
US9040297B2 · kind B2 · utility
12Cited by
28References
48Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 2, 2007 |
| Grant date | May 26, 2015 |
| Priority date | — |
| Expiry date | Nov 14, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/90
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of expanding and maintaining human embryonic stem cells (ESCs) in an undifferentiated state by culturing the ESCs in a suspension culture under culturing conditions devoid of substrate adherence is provided. Also provided are a method of deriving ESC lines in the suspension culture and methods of generating lineage-specific cells from ESCs which were expanded in the suspension culture of the present invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.