Controlled cross-linking processing of proteins
US9040665B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 26, 2012 |
| Grant date | May 26, 2015 |
| Priority date | — |
| Expiry date | Mar 26, 2032 |
Classification
- Technology area (CPC D)Textiles; Paper
- CPC primaryD01F4/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of forming a cross-linked protein structures includes preparing a solution of protein dissolved in a benign solvent and forming an intermediate protein structure from the solution. The intermediate protein structure can be cross-linked by providing for a specific ratio of chemical cross-linking agents to form the cross-linked protein structure. The solution can be prepared by adding a cross-linker of N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) at a ratio of two-to-one of NHS to EDC to alcohol. PBS buffer (20×) can be added to the solution until the volume ratio of PBS buffer (20×) to alcohol is about one-to-one. About 16 percent by weight of protein can be dissolved in the solution. The solution can be electrospun to form an intermediate protein structure. After a period of time, the protein structure can be cross-linked to form the cross-linked protein structure.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.