Cloning, expression and use of acid phospholipases

US9045713B2 · kind B2 · utility

Assignee

• AB Enzymes GmbH · DE

Inventors

• Khanh Q. Nguyen · Scottsdale, US
• Kornelia Titze · Heilbad Heiligenstadt, DE
• Tatiana Schwarz · Langen (Hessen), DE
• Silvia Paladino · Pappenheim, DE
• Volker Marschner · Emmelshausen, DE
• Patrick Lorenz · Lorsch, DE

Key dates

• Filing date: Oct 27, 2010
• Grant date: Jun 2, 2015
• Expiry date: Jan 17, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y301/01032
• WIPO field: Food chemistry
• WIPO sector: Chemistry

Abstract

The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID NO: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for by the plasmid pPL3940-Topo2.5 with the restriction map according to FIG. 7, which is deposited under accession number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) due to the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA seque…