Devices and methods for integrated continuous manufacturing of biological molecules
US9045725B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 30, 2005 |
| Grant date | Jun 2, 2015 |
| Priority date | — |
| Expiry date | Mar 31, 2028 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01D2317/04
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The present invention relates to a process and apparatus for purifying a molecule of interest from a heterogeneous clarified fluid mixture. The apparatus of the invention generally comprises a continuous perfusion fermentation system, a continuous particle removal system integrated with the perfusion fermentation system; and a continuous purification system integrated with the particle removal system, which is maintained under sterile conditions. The process comprises filtering a heterogeneous clarified fluid mixture by continuous ultrafiltration at a specific flow rate below the transition point of the molecule of interest in the pressure-dependent region of the flux versus TMP curve, wherein the specific flow rate is maintained substantially constant throughout the continuous ultrafiltration.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.