Patent · US Active

Inkjet gene printing

US9045757B2 · kind B2 · utility

2Cited by

7References

16Claims

0Family size

Assignee

Wake Forest University Health Sciences · US

Inventors

Tao Xu · Hangzhou City, CN
James J. Yoo · Winston-Salem, US
Anthony Atala · Winston-Salem, US

Key dates

Filing date	Jun 6, 2008
Grant date	Jun 2, 2015
Priority date	—
Expiry date	Mar 11, 2030

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/89
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

Provided herein are methods and apparatuses for transfecting a cell with a compound of interest by stressing the cell, e.g. with shear stress. The compound of interest may be nucleic acids, proteins, molecules, nanoparticles, drugs, etc., or any combination thereof. Methods of printing cells with an inkjet printing device are also provided, wherein at least a portion of viable cells (preferably at least 1%) are transfected with a compound of interest. Preferably, at least 25% of the cells are viable after printing. In addition, methods of forming an array of viable cells are provided wherein at least a portion of the viable printed cells (preferably at least 1%) are transfected with at least one compound of interest.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.