Droplet actuator devices and methods employing magnetic beads
US9046514B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 11, 2008 |
| Grant date | Jun 2, 2015 |
| Priority date | — |
| Expiry date | Nov 5, 2029 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2035/1046
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator (200) comprising: (i) a substrate (210) comprising electrodes (214) arranged for conducting droplet operations on a surface; (ii) a starting droplet (218/222) comprising: (1) one or more magnetically responsive beads (220); (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone, (224); (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet (218) comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet (222) substantially lacking the magnetically responsive beads.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.