Alkali-resistant variants of protein A and their use in affinity chromatography
US9051375B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Dec 21, 2011 |
| Grant date | Jun 9, 2015 |
| Priority date | — |
| Expiry date | Dec 21, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K17/06
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The present invention relates to immunoglobulin (Ig)-binding proteins with alkali-resistance properties. In one embodiment, the present invention provides for a variant of an Ig-binding protein, the variant comprising the Ig-binding protein having at least one asparagine residue substituted with a histidine, a serine, an aspartic acid or a threonine residue. The at least one substitution may confer to the variant Ig-binding protein an increased stability in alkaline solutions when compared to the wild-type Ig-binding. The present invention relates also to matrices for affinity separation of immunoglobulins comprising the Ig-binding proteins of the present invention, and to methods of using the Ig-binding proteins of the present invention to separate immunoglobulins from mixture compositions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.