In situ tissue engineering using magnetically guided three dimensional cell patterning
US9051549B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 22, 2012 |
| Grant date | Jun 9, 2015 |
| Priority date | — |
| Expiry date | Nov 19, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/74
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Methods are provided for the three dimensional manipulation of cells, and for the formation of an organized engineered cell tissue. Also provided are the organized engineered cell tissues produced by the methods. In one method, a plurality of magnetically labeled cells are mixed with a cross-linkable hydrogel to form a cell-hydrogel mixture, the at least a portion of the plurality of magnetically labeled cells are manipulated with a magnetic field to arrange the magnetically labeled cells into a specific cellular arrangement, and the hydrogel is crosslinked to form the organized engineered cell tissue. The approach presented herein offers a means to circumvent the deficiencies in the field of regenerative medicine, and allows for the production of organized tissues in situ with specific cellular organizations that mimic the native tissue.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.