Patent · US Active

Detection and quantification of biomolecules using mass spectrometry

US9051608B2 · kind B2 · utility

0Cited by

108References

18Claims

0Family size

Assignee

AGENA BIOSCIENCE, INC. · US

Inventors

Dirk Johannes Van Den Boom · Dreieich, DE
Paul Andrew Oeth · San Diego, US
Payam Mahboubi · San Diego, US

Key dates

Filing date	Feb 13, 2013
Grant date	Jun 9, 2015
Priority date	—
Expiry date	Feb 14, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6883
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry. This process is easily incorporated into a polymerase chain reaction (PCR) amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.