Microarray synthesis and assembly of gene-length polynucleotides
US9051666B2 · kind B2 · utility
77Cited by
105References
28Claims
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Key dates
| Filing date | Sep 14, 2012 |
| Grant date | Jun 9, 2015 |
| Priority date | — |
| Expiry date | Sep 14, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B40/06
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.