Detection and enumeration of microorganisms

US9057093B2 · kind B2 · utility

Assignees

• BASF SE
• CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE · FR

Inventors

• Yannick Fovet · Edingen-Neckarhausen, DE
• Adrien Ducret · Bloomington, US
• Sam Dukan · Marseilles, FR
• Marina Periame · Marseilles, FR

Key dates

• Filing date: Jun 1, 2011
• Grant date: Jun 16, 2015
• Expiry date: Jun 1, 2031

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for detecting and enumerating viable microorganisms in a sample suspected of containing said microorganisms: (1) contacting said microorganisms of said sample with repair compounds and a growth medium, and (2) incubating the product of step (1),and (3) detecting and enumerating said microorganisms, in which the microorganisms are of the species Legionella pneumophila, and in which the repair compounds comprise: (a)serine;(b)threonine;(c)a compound containing calcium ions at a dose of 10−6 to 10−2 mM;(d)a compound containing magnesium ions at a dose of 10−6 to 10−2 mM;(e)a compound containing potassium ions;(f) glutamic acid or a salt thereof; and (g)pyruvic acid or a salt thereof. The invention also discloses a kit for detecting and enumerating viable microorganisms of the species Legionella pneumophila in a sample suspected of containing said microorganisms.