Enhanced on-chip SERS based biomolecular detection using electrokinetically active microwells
US9057099B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 14, 2009 |
| Grant date | Jun 16, 2015 |
| Priority date | — |
| Expiry date | Jul 15, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2565/632
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for detecting target nucleic acids such as SNPs is provided. The method comprises performing a ligase detection reaction (LDR), performing surface enhanced Raman scattering (SERS) on the LDR, and analyzing the outcome of the LDR using analysis and/or quantification of the SERS by detecting an emitted Raman signature. The LDR-SERS method can be used for sensitive and specific detection of any nucleic acid sequence of interest. A microfluidic SERS detection device is also provided. The device comprises electrokinetically active microwells for mixing and concentrating analytes and in which analytes can be quantified. The device can be used for performing the LDR-SERS method in optofluidic chip format.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.