Polymerase enzyme substrates with protein shield
US9062091B2 · kind B2 · utility
97Cited by
25References
21Claims
0Family size
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Key dates
| Filing date | Feb 14, 2013 |
| Grant date | Jun 23, 2015 |
| Priority date | — |
| Expiry date | Feb 25, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2563/131
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Compositions and methods are provided for nucleotide analogs comprising protein shields for improving enzyme photostability in single molecule real time sequencing. Nucleotide analogs of the invention have a protein shield between the dye moieties and nucleotide moieties of the analog. The protein prevents the direct interaction of the dye moiety with the enzyme carrying out nucleotide synthesis preventing photodamage to the enzyme. The nucleotide analogs of the invention can have multiple dyes and multiple nucleotide moieties.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.