Patent · US Active

Methods and compositions for silencing gene families using artificial microRNAs

US9062317B2 · kind B2 · utility

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Key dates

Filing dateMay 3, 2012
Grant dateJun 23, 2015
Priority date—
Expiry dateJan 11, 2033

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N15/8247
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Methods and compositions are provided which allow for a single microRNA (miRNA) to reduce the level of expression of at least two members of the same protein and/or gene family. Such methods and compositions employ miRNA expression constructs having a structure such that the most abundant form of miRNA produced from the construct is a 22-nucleotide miRNA. The 22-nucleotide miRNA produced from the miRNA expression construct thereby reduces the level of expression of not only the target sequence for the miRNA, but also reduces the level of expression of at least one additional sequence from the same protein and/or gene family as the target sequence.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.