Processes for synthesizing DNA with non-standard nucleotides
US9062345B1 · kind B1 · utility
Inventors
Key dates
| Filing date | Jan 15, 2014 |
| Grant date | Jun 23, 2015 |
| Priority date | — |
| Expiry date | Jan 15, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2525/117
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The disclosed invention provides processes to synthesize DNA analogs that contain non-standard nucleotides, defined as those which form nucleobase pairs that fit standard Watson-Crick geometry, but are joined to their complements by hydrogen bonding patterns different from those that join standard A:T and G:C pairs. The disclosed process resembles “rolling circle amplification”, but uses primers that contain non-standard nucleotides, as well as 2′-deoxynucleotide triphosphates whose heterocyclic “nucleobases” are also non-standard. An example is provided that shows this process using 6-amino-5-nitro-3-(1′-beta-D-2′-deoxyribofuranosyl)-2(1H)-pyridone to implement the non-standard “small” donor-donor-acceptor (pyDDA) hydrogen bonding pattern, and 2-amino-8-(1′-beta-D-2′-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one to implement the “large” acceptor-acceptor-donor (puAAD) pattern.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.