Optical approach for microfluidic DNA electrophoresis detection
US9067207B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Mar 4, 2011 |
| Grant date | Jun 30, 2015 |
| Priority date | — |
| Expiry date | Jan 9, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/4186
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Aspects of the disclosure provides a DNA analyzer to facilitate an integrated single-chip DNA analysis. The DNA analyzer includes an interface for coupling a microfluidic chip to the DNA analyzer. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, and a second domain fluidically coupled to the first domain to receive the DNA fragments and perform electrophoretic separation of the DNA fragments. The DNA fragments are tagged with fluorescent labels. The DNA analyzer includes a detection module to excite the fluorescent labels to emit fluorescence and detect the emitted fluorescence. The detection module includes a laser source, a set of optical elements, a filter module and a photo-detector.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.