Cardiac differentiation of human pluripotent stem cells under defined conditions using matrix overlay methods
US9068167B2 · kind B2 · utility
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23Claims
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Key dates
| Filing date | Nov 15, 2010 |
| Grant date | Jun 30, 2015 |
| Priority date | — |
| Expiry date | Feb 26, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2506/45
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for culturing the pluripotent stem cells to undergo epithelial-to-mesenchymal transition and for generating high-yield, high-purity cardiomyocyte cultures from pluripotent stem cells are described. Pluripotent stem cells are cultured on a support with an overlaid matrix and, optionally, exposed to one or more factors to induce epithelial-to-mesenchymal transition and cardiogenesis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.