Ligation method employing RtcB
US9074203B2 · kind B2 · utility
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19Claims
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Key dates
| Filing date | Feb 8, 2012 |
| Grant date | Jul 7, 2015 |
| Priority date | — |
| Expiry date | Dec 17, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y605/01004
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of processing an RNA sample is provided. In certain embodiments, the method may comprise: a) obtaining a fragmented RNA sample comprising: i. RNA fragments of long RNA molecules; and ii. unfragmented short RNA; and b) contacting said fragmented RNA sample with a first adaptor in the presence of a RtcB ligase, thereby producing a ligated RNA sample comprising adaptor-ligated fragments of long RNA. A kit for performing the method is also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.