Apparatus and methods for microbial identification by mass spectrometry

US9074236B2 · kind B2 · utility

Assignee

• OXOID LIMITED · GB

Inventors

• James L. Stephenson, Jr. · Raleigh, US
• Oksana Gvozdyak · Bedford, US
• Roger Grist · Crowborough, GB
• Clay Campbell · San Jose, US
• Ian D. Jardine · Los Gatos, US
• Iain C. Mylchreest · Santa Clara, US

Key dates

• Filing date: Apr 30, 2013
• Grant date: Jul 7, 2015
• Expiry date: Apr 30, 2033

Classification

• Technology area (CPC H): Electricity
• CPC primary: H01J49/165
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods and systems for identification of microorganisms either after isolation from a culture or directly from a sample. The methods and systems are configured to identify microorganisms based on the characterization of proteins of the microorganisms via high-resolution/mass accuracy single-stage (MS) or multi-stage (MSn) mass spectrometry. Included herein are also discussion of targeted detection and evaluation of virulence factors, antibiotic resistance markers, antibiotic susceptibility markers, typing, or other characteristics using a method applicable to substantially all microorganisms and high-resolution/mass accuracy single-stage (MS) or multi-stage (MSn) mass spectrometry.