CDR-anchored amplification method
US9079942B2 · kind B2 · utility
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23References
17Claims
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Key dates
| Filing date | Jan 28, 2010 |
| Grant date | Jul 14, 2015 |
| Priority date | — |
| Expiry date | Sep 19, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6881
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of obtaining nucleic acid encoding a plurality of antibodies is provided. In certain embodiments, the method comprises obtaining from an immunized animal nucleic acid encoding the amino acid sequence of the heavy and light chains of a second antibody that binds to the antigen as a first antibody and differs in amino acid sequence to the first antibody, wherein the obtaining is done by amplification using: i. a first primer pair that includes oligonucleotides are complementary to CDR-encoding regions first antibody.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.