Culturing human embryonic stem cells with a noggin to generate cells lacking Pax-6 expression
US9080147B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jan 30, 2009 |
| Grant date | Jul 14, 2015 |
| Priority date | — |
| Expiry date | Apr 13, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2506/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a preparation of undifferentiated embryonic stem (ES) cells sustainable for a prolonged period in an undifferentiated state which will undergo stem cell renewal or somatic differentiation. Preferably the cells are capable of somatic differentiation in vitro and are inclined to differentiate away from an extraembryonic lineage. The present invention also provides method of culturing embryonic stem (ES) cells to improve stem cell maintenance and persistence in culture. The method also provides a culture of ES cells prepared by the method as well as differentiated cells derived from the embryonic cells resulting from directed differentiation procedures provided by the present invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.