Method for determining equivalent thermal conditions between liquid chromatography systems
US9086424B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 21, 2011 |
| Grant date | Jul 21, 2015 |
| Priority date | — |
| Expiry date | May 13, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/3007
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Described is a method of transferring a chromatographic method between liquid chromatography (LC) systems. The method is based on a determination of an isoretention temperature at which two solutes co-elute. The method enables separations to be performed using different LC systems with reproducible and equivalent results. For example, the method allows for a chromatography method developed for HPLC to be more readily transferred to a UPLC system and for a chromatography method developed for a UPLC system to be more readily transferred to a HPLC system. The method addresses LC systems having column ovens of different design in which the internal column temperatures are not equal although the operating temperatures of the column ovens may be accurately controlled to equal values. The retention behavior and resolution of different LC systems is caused to be substantially the same so that equivalent separation results are obtained.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.