DNA amplification apparatus and method
US9089851B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Nov 15, 2012 |
| Grant date | Jul 28, 2015 |
| Priority date | — |
| Expiry date | Feb 19, 2033 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/1872
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The invention relates to a method for the amplification of DNA and apparatus for such amplification. The method comprises steps typically found in amplification methods but utilizes an optical procedure to detect denaturation of DNA or attainment of the desired denaturation temperature. The apparatus (1) comprises a temperature controllable chamber (2) including a rotor (3) for holding a plurality of reaction vessels (see 7 for example) for reaction mixtures including DNA, a drive means for the rotor, a heater (8) within the chamber for transiently supplying infrared energy to the reaction vessels, and an optical system (12-16) for determining denaturation of at least a reference DNA or for detecting attainment of a desired denaturation temperature in at least a reference reaction vessel.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.