Rapid isolation of monoclonal antibodies from animals

US9090674B2 · kind B2 · utility

Assignee

• The Board of Regents of the University of Texas System · US

Inventors

• Sai Reddy · Basel, CH
• Xin Ge · Riverside, US
• Jason Lavinder · Round Rock, US
• Daniel BOUTZ · Austin, US
• Andrew Ellington · Austin, US
• Edward MARCOTTE · Austin, US
• George Georgiou · Austin, US

Key dates

• Filing date: May 17, 2011
• Grant date: Jul 28, 2015
• Expiry date: Jan 20, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B30/04
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods and compositions for identification of candidate antigen-specific variable regions as well as generation of antibodies or antigen-binding fragments that could have desired antigen specificity are provided. For example, in certain aspects methods for determining amino acid sequences of serum antibody CDR and abundancy level are described. In some aspects, methods for determining nucleic acid sequences of antibody variable region sequences and frequency are provided. Furthermore, the invention provides methods for identification and generation of antibody or antigen-binding fragments that comprise highly-represented CDR.