Variant alpha-amylases from Bacillus subtilis and methods of use, thereof

US9090887B2 · kind B2 · utility

Assignee

• Danisco US Inc. · US

Inventors

• William A. Cuevas · San Francisco, US
• Sang-Kyu Lee · Palo Alto, US
• Sandra W. Ramer · Palo Alto, US
• Andrew Shaw · San Francisco, US
• Amr R. Toppozada · San Francisco, US
• David E Estell · San Francisco, US
• Louise Wallace · Redwood City, US
• Regina Chin · Palo Alto, US
• Carol A. Requadt · Paradise, US
• Scott D. Power · San Bruno, US
• Michael J. Pepsin · Castro Valley, US

Key dates

• Filing date: Jun 5, 2009
• Grant date: Jul 28, 2015
• Expiry date: Aug 5, 2030

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/10
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.